Ploidy and genome composition of Musa germplasm at the International Institute of Tropical Agriculture (IITA)

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cg.authorship.typesCGIAR and advanced research institute
cg.contributor.affiliationCzech Academy of Sciences
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.countryNigeria
cg.coverage.countryUganda
cg.coverage.iso3166-alpha2NG
cg.coverage.iso3166-alpha2UG
cg.coverage.regionAfrica
cg.coverage.regionWestern Africa
cg.coverage.regionEastern Africa
cg.issn1684-5315
cg.issue13
cg.reviewStatusPeer Review
cg.subject.iitaGENETIC IMPROVEMENT
cg.subject.iitaPLANT BREEDING
cg.subject.iitaBANANA
cg.subject.iitaPLANTAIN
cg.volume5
dc.contributor.authorPillay, M.
dc.contributor.authorOgundiwin, E.
dc.contributor.authorTenkouano, A.
dc.contributor.authorDoležel, Jaroslav
dc.date.accessioned2018-03-23T06:48:45Zen
dc.date.available2018-03-23T06:48:45Zen
dc.identifier.urihttps://hdl.handle.net/10568/91780
dc.titlePloidy and genome composition of Musa germplasm at the International Institute of Tropical Agriculture (IITA)en
dcterms.abstractMusa spp (bananas and plantains) constitute a hybrid-polyploid complex and are classified according to different genome compositions such as AA, BB, AB, AAA, AAB, ABB, AAAA, ABBB, AAAB and AABB. Knowledge of ploidy and exact genome compositions of the parental material is essential for Musa breeding. This study determined the ploidy levels and genome composition of the Musa germplasm collection, constituting over 300 accessions, at the International Institute of Tropical Agriculture in Nigeria and Uganda. Flow cytometric analysis of nuclear DNA content was used to estimate ploidy levels, while genome composition was ascertained with RAPD markers that are specific for the A and B genomes of Musa. It was determined that at least 8% of the plants in the germplasm collection were miss-classified in terms of ploidy and/or genome composition. The cultivars 'Pisang awak', 'Foulah 4' and 'Nzizi', previously classified as triploids, were found to be tetraploids by flow cytometry and conventional root tip chromosome counts. Similarly, cultivars that were previously classified as diploids including 'Too', and 'Toowoolee' were found to be triploids in our analysis. Ploidy and genome classification in Musa was generally determined from morphological characteristics. While our study showed that such a system is not always reliable, it was interesting to find that none of the plantains in the germplasm collection were miss-classified with regards to both ploidy and genome composition.en
dcterms.accessRightsOpen Access
dcterms.bibliographicCitationPillay, M., Ogundiwin, E., Tenkouano, A. & Dolezel, J. (2006). Ploidy and genome composition of Musa germplasm at the International Institute of Tropical Agriculture (IITA). African Journal of Biotechnology, 5(13), 1224-1232.en
dcterms.extentp. 1224-1232
dcterms.issued2006
dcterms.languageen
dcterms.subjectbananasen
dcterms.subjectplantainsen
dcterms.subjectgenomesen
dcterms.subjectploiden
dcterms.typeJournal Article

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