Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses

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cg.authorship.typesCGIAR and advanced research institute
cg.contributor.affiliationInternational Potato Center
cg.contributor.affiliationSwedish University of Agricultural Sciences
cg.howPublishedFormally Published
cg.identifier.dataurlhttp://doi.org/10.21223/p3/nkwpgeen
cg.identifier.doihttps://doi.org/10.1016/j.virol.2009.03.024
cg.isijournalISI Journal
cg.issn0042-6822
cg.issue1
cg.journalVirology
cg.reviewStatusPeer Review
cg.volume388
dc.contributor.authorKreuze, Jan F.
dc.contributor.authorPérez, A.
dc.contributor.authorUntiveros, M.
dc.contributor.authorQuispe, D.
dc.contributor.authorFuentes, S.
dc.contributor.authorBarker, I.
dc.contributor.authorSimon, R.
dc.date.accessioned2017-02-07T13:03:33Zen
dc.date.available2017-02-07T13:03:33Zen
dc.identifier.urihttps://hdl.handle.net/10568/79776
dc.titleComplete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of virusesen
dcterms.abstractWe report the first identification of novel viruses, and sequence of an entire viral genome, by a single step of high-throughput parallel sequencing of small RNAs from diseased, as well as symptomless plants. Contigs were assembled from sequenced total siRNA from plants using small sequence assembly software and could positively identify RNA, ssDNA and dsDNA reverse transcribing viruses and in one case spanned the entire genome. The results present a novel approach which cannot only identify known viral pathogens, occurring at extremely low titers, but also novel viruses, without the necessity of any prior knowledge.en
dcterms.accessRightsLimited Access
dcterms.audienceScientists
dcterms.bibliographicCitationKreuze, J.F.; Perez, A.; Untiveros, M.; Quispe, D.; Fuentes, S.; Barker, I.; Simon, R. 2009. Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses. Virology. (USA). ISSN 0042-6822. 388(1):1-7.en
dcterms.extent1-7
dcterms.issued2009-05
dcterms.languageen
dcterms.licenseCopyrighted; all rights reserved
dcterms.publisherElsevier
dcterms.subjectgenomesen
dcterms.subjectvirosesen
dcterms.subjectdiagnosisen
dcterms.subjectplant virusesen
dcterms.subjectsweet potatoesen
dcterms.typeJournal Article

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