Two Novel DNAs that enhance symptoms and overcome CMD2 resistance to cassava mosaic disease

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cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationInternational Center for Tropical Agriculture
cg.coverage.countryTanzania
cg.coverage.iso3166-alpha2TZ
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.creator.identifierJoe Tohme: 0000-0003-2765-7101
cg.identifier.doihttps://doi.org/10.1128/jvi.02834-15
cg.isijournalISI Journal
cg.issn0022-538X
cg.issue8
cg.journalJournal of Virology
cg.reviewStatusPeer Review
cg.subject.ciatCASSAVA
cg.subject.ciatPESTS AND DISEASES
cg.subject.ciatPLANT BREEDING
cg.volume90
dc.contributor.authorNdunguru, Joseph
dc.contributor.authorLeón, Leandro de
dc.contributor.authorDoyle, Catherine D.
dc.contributor.authorSseruwagi, Peter
dc.contributor.authorPlata, Germán
dc.contributor.authorLegg, James P.
dc.contributor.authorThompson, Graham
dc.contributor.authorTohme, Joseph M.
dc.contributor.authorAveling, Theresa
dc.contributor.authorAscencio Ibáñez, Jose T.
dc.contributor.authorHanley Bowdoin, Linda
dc.date.accessioned2016-05-19T20:34:19Zen
dc.date.available2016-05-19T20:34:19Zen
dc.identifier.urihttps://hdl.handle.net/10568/73456
dc.titleTwo Novel DNAs that enhance symptoms and overcome CMD2 resistance to cassava mosaic diseaseen
dcterms.abstractCassava mosaic begomoviruses (CMBs) cause cassava mosaic disease (CMD) across Africa and the Indian subcontinent. Like all members of the geminivirus family, CMBs have small, circular single-stranded DNA genomes. We report here the discovery of two novel DNA sequences, designated SEGS-1 and SEGS-2 (for sequences enhancing geminivirus symptoms), that enhance symptoms and break resistance to CMD. The SEGS are characterized by GC-rich regions and the absence of long open reading frames. Both SEGS enhanced CMD symptoms in cassava (Manihot esculenta Crantz) when coinoculated with African cassava mosaic virus (ACMV), East African cassava mosaic Cameroon virus (EACMCV), or East African cassava mosaic virus-Uganda (EACMV-UG). SEGS-1 also overcame resistance of a cassava landrace carrying the CMD2 resistance locus when coinoculated with EACMV-UG. Episomal forms of both SEGS were detected in CMB-infected cassava but not in healthy cassava. SEGS-2 episomes were also found in virions and whiteflies. SEGS-1 has no homology to geminiviruses or their associated satellites, but the cassava genome contains a sequence that is 99% identical to full-length SEGS-1. The cassava genome also includes three sequences with 84 to 89% identity to SEGS-2 that together encompass all of SEGS-2 except for a 52-bp region, which includes the episomal junction and a 26-bp sequence related to alphasatellite replication origins. These results suggest that SEGS-1 is derived from the cassava genome and facilitates CMB infection as an integrated copy and/or an episome, while SEGS-2 was originally from the cassava genome but now is encapsidated into virions and transmitted as an episome by whiteflies.en
dcterms.accessRightsOpen Access
dcterms.audienceScientists
dcterms.bibliographicCitationNdunguru, Joseph; De León, Leandro; Doyle, Catherine D.; Sseruwagi, Peter; Plata, German; Legg, James P.; Thompson, Graham; Tohme, Joseph M.; Aveling, Theresa; Ascencio Ibáñez, Jose T.; Hanley Bowdoin, Linda. 2016. Two Novel DNAs that enhance symptoms and overcome CMD2 resistance to cassava mosaic disease . Journal of Virology 90(8): 4160-4173.en
dcterms.extent4160-4173
dcterms.issued2016-04-15
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherAmerican Society for Microbiology
dcterms.subjectmanihot esculentaen
dcterms.subjectafrican cassava mosaic virusen
dcterms.subjectplant diseasesen
dcterms.subjectwhitefliesen
dcterms.subjectdisease resistanceen
dcterms.subjectgenetic resistanceen
dcterms.subjectplant breedingen
dcterms.subjectvirus del mosaico de la cassavaen
dcterms.subjectenfermedades de las plantasen
dcterms.subjectresistencia a la enfermedaden
dcterms.subjectresistencia genéticaen
dcterms.subjectfitomejoramientoen
dcterms.typeJournal Article

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