In vitro tuberization in white yam (Dioscorea rotundata Poir)

Ng, S.Y.C. (1988). In vitro tuberization in white yam (Dioscorea rotundata Poir). Plant cell, tissue and organ culture, 14(2), 121-128.

Nodal cuttings of white yam were induced to produce microtubers on a MS-revised medium supplemented with various concentrations of sucrose, 20 mgl−1 L-cysteine, 0.5 mgl−1 kinetin and 0.7% agar. The frequency of tuberization was affected by the daylength, which is optimal at 12 and 16 h of light depending on the sucrose concentration. The microtubers were planted in a seed bed and grown to maturity. The importance of in vitro tuberization of yam as a means of international germplasm distribution or exchange as well as for the propagation of planting material is discussed.