Comprehensive analysis of differentially expressed mRNA profiles in chicken jejunum and cecum following Eimeria maxima infection

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cg.authorship.typesCGIAR and developing country instituteen
cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationSouth China Agricultural Universityen
cg.contributor.affiliationMinistry of Agriculture, Chinaen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationLanzhou Universityen
cg.contributor.affiliationUniversity of Nottinghamen
cg.contributor.donorNatural Scientific Foundation of Chinaen
cg.contributor.donorBill & Melinda Gates Foundationen
cg.contributor.donorForeign, Commonwealth and Development Office, United Kingdomen
cg.contributor.donorChinese Governmenten
cg.coverage.countryEthiopia
cg.coverage.iso3166-alpha2ET
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.creator.identifierOlivier Hanotte: 0000-0002-2877-4767
cg.creator.identifierEndashaw Jebessa: 0000-0002-2759-6663
cg.creator.identifierMekonnen Girma Nedi: 0000-0002-8645-8359
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1016/j.psj.2024.103716en
cg.isijournalISI Journalen
cg.issn1525-3171en
cg.issue6en
cg.journalPoultry Scienceen
cg.reviewStatusPeer Reviewen
cg.species.breedHORROen
cg.subject.ilriANIMAL BREEDINGen
cg.subject.ilriBREEDSen
cg.subject.ilriGENETICSen
cg.subject.ilriPOULTRYen
cg.subject.ilriCHICKENSen
cg.volume103en
dc.contributor.authorJebessa, Endashawen
dc.contributor.authorBello, S.F.en
dc.contributor.authorYibin Xuen
dc.contributor.authorCai, B.en
dc.contributor.authorDaba, M.en
dc.contributor.authorGirma, Mekonnenen
dc.contributor.authorBordbar, F.en
dc.contributor.authorHanotte, Olivier H.en
dc.contributor.authorQinghua Nieen
dc.date.accessioned2024-07-02T04:34:28Zen
dc.date.available2024-07-02T04:34:28Zen
dc.identifier.urihttps://hdl.handle.net/10568/148830
dc.titleComprehensive analysis of differentially expressed mRNA profiles in chicken jejunum and cecum following Eimeria maxima infectionen
dcterms.abstractCoccidiosis, a protozoan disease that substantially impacts poultry production, is characterized by an intracellular parasite. The study utilized 48 one-day-old Horro chickens, randomly divided into the infected (I) and control (C) groups. The challenge group of chickens were administered Eimeria maxima oocysts via oral gavage at 21-days-old, and each chicken received 2 mL containing 7×104 sporulated oocysts. The total RNAs of chicken jejunum and cecum tissues were isolated from three samples, each from I and C groups. Our study aimed to understand the host immune-parasite interactions and compare immune response mRNA profiles in chicken jejunum and cecum tissues at 4 and 7 days postinfection with Eimeria maxima. The results showed that 823 up- and 737 down-regulated differentially expressed mRNAs (DEmRNAs) in jejunum at 4 d infection and control (J4I vs. J4C), and 710 up- and 368 down-regulated DEmRNAs in jejunum at 7 days infection and control (J7I vs. J7C) were identified. In addition, DEmRNAs in cecum tissue, 1424 up- and 1930 down-regulated genes in cecum at 4 days infection and control (C4I vs. C4C), and 77 up- and 191 down-regulated genes in cecum at 7 days infection and control (C7I vs. C7C) were detected. The crucial DEmRNAs, including SLC7A5, IL1R2, GLDC, ITGB6, ADAMTS4, IL1RAP, TNFRSF11B, IMPG2, WNT9A, and FOXF1, played pivotal roles in the immune response during Eimeria maxima infection of chicken jejunum. In addition, the potential detection of FSTL3, RBP7, CCL20, DPP4, PRKG2, TFPI2, and CDKN1A in the cecum during the host immune response against Eimeria maxima infection is particularly noteworthy. Furthermore, our functional enrichment analysis revealed the primary involvement of DEmRNAs in small molecule metabolic process, immune response function, inflammatory response, and toll-like receptor 10 signaling pathway in the jejunum at 4 and 7 days postinfection. Similarly, in the cecum, DEmRNAs at 4 and 7 days postinfection were enriched in processes related to oxidative stress response and immune responses. Our findings provide new insights and contribute significantly to the field of poultry production and parasitology.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.available2024-06-15
dcterms.bibliographicCitationJebessa, E., Bello, S.F., Yibin Xu, Cai, B., Daba, M., Girma, M., Bordbar, F., Hanotte, O. and Qinghua Nie. 2024. Comprehensive analysis of differentially expressed mRNA profiles in chicken jejunum and cecum following Eimeria maxima infection. Poultry Science 103(6):103716.en
dcterms.issued2024-06-15
dcterms.languageen
dcterms.licenseCC-BY-NC-ND-4.0
dcterms.publisherElsevieren
dcterms.subjectanimal breedingen
dcterms.subjectgeneticsen
dcterms.subjectchickensen
dcterms.subjectpoultryen
dcterms.subjectbreedsen
dcterms.subjectEimeria maximaen
dcterms.subjectjejunumen
dcterms.subjectcecumen
dcterms.typeJournal Article

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