Heterologous expression of the Haynaldia villosa pattern-recognition receptor CERK1-V in wheat increases resistance to three fungal diseases

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cg.authorship.typesCGIAR and developing country institute
cg.authorship.typesCGIAR and advanced research institute
cg.contributor.affiliationNanjing Agricultural University
cg.contributor.affiliationCornell University
cg.contributor.affiliationInternational Maize and Wheat Improvement Center
cg.contributor.donorNational Natural Science Foundation of China
cg.contributor.donorNational Key Research and Development Program, China
cg.contributor.donorChina Postdoctoral Science Foundation
cg.contributor.donorCGIAR Trust Fund
cg.contributor.initiativePlant Health
cg.creator.identifierRavi Prakash Singh: 0000-0002-4676-5071
cg.howPublishedFormally Published
cg.identifier.doihttps://doi.org/10.1016/j.cj.2022.02.005
cg.isijournalISI Journal
cg.issn2214-5141
cg.issue6
cg.journalThe Crop Journal
cg.placeNetherlands
cg.reviewStatusPeer Review
cg.subject.actionAreaResilient Agrifood Systems
cg.subject.impactAreaNutrition, health and food security
cg.volume10
dc.contributor.authorAnqi Fan
dc.contributor.authorLuyang Wei
dc.contributor.authorXu Zhang
dc.contributor.authorJia Liu
dc.contributor.authorLi Sun
dc.contributor.authorJin Xiao
dc.contributor.authorYajia Wang
dc.contributor.authorHaiyan Wang
dc.contributor.authorJian Hua
dc.contributor.authorSingh, Ravi P.
dc.contributor.authorZongkuan Wang
dc.contributor.authorXiue Wang
dc.date.accessioned2023-04-23T10:57:00Zen
dc.date.available2023-04-23T10:57:00Zen
dc.identifier.urihttps://hdl.handle.net/10568/130125
dc.titleHeterologous expression of the Haynaldia villosa pattern-recognition receptor CERK1-V in wheat increases resistance to three fungal diseasesen
dcterms.abstractWheat production is under continuous threat by various fungal pathogens. Identification of multiple-disease resistance genes may lead to effective disease control via the development of cultivars with broad-spectrum resistance. Plant Lysin-motif (LysM)-type pattern-recognition receptors, which elicit innate immunity by recognizing fungal pathogen associated molecular patterns such as chitin, are potential candidates for such resistance. In this study, we cloned a LysM receptor-like kinase gene, CERK1-V, from the diploid wheat relative Haynaldia villosa. CERK1-V expression was induced by chitin and Blumeria graminis f. sp. tritici, the causal agent of wheat powdery mildew. Heterologous overexpression of CERK1-V in wheat inhibited the development of three fungal pathogens, thereby increased resistance to powdery mildew, yellow rust, and Fusarium head blight. CERK1-V physically interacted with the wheat LysM protein TaCEBiPs. CERK1-V/TaCEBiPs interaction promoted chitin recognition and activated chitin signal transduction in wheat. Transgenic plants with excessively high CERK1-V expression showed high resistance but abnormal plant growth, whereas plants with moderate expression level showed adequate resistance level with no marked impairment of plant growth. In transgenic lines, RNA-seq showed that gene expression involved in plant innate immunity was activated. Expression of genes involved in photosynthesis, ER stress and multiple phytohormone pathways was also activated. Optimized expression of CERK1-V in wheat can confer disease resistance without compromising growth or defense fitness.en
dcterms.accessRightsOpen Access
dcterms.audienceScientists
dcterms.bibliographicCitationFan, A., Wei, L., Zhang, X., Liu, J., Sun, L., Xiao, J., Wang, Y., Wang, H., Hua, J., Singh, R. P., Wang, Z., & Wang, X. (2022). Heterologous expression of the Haynaldia villosa pattern-recognition receptor CERK1-V in wheat increases resistance to three fungal diseases. The Crop Journal, 10(6), 1733–1745. https://doi.org/10.1016/j.cj.2022.02.005en
dcterms.extentpp. 1733-1745
dcterms.issued2022-12
dcterms.languageen
dcterms.licenseCC-BY-NC-ND-4.0
dcterms.publisherElsevier
dcterms.subjectfungal diseasesen
dcterms.subjectdasypyrum villosumen
dcterms.subjectwheaten
dcterms.subjectdisease resistanceen
dcterms.typeJournal Article

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