Marker development for quality protein maize breeding and an interaction study between Opaque-2 and Ask2 genes

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cg.authorship.typesCGIAR and developing country institute
cg.contributor.affiliationColegio de Postgraduados, Mexico
cg.contributor.affiliationInternational Maize and Wheat Improvement Center
cg.contributor.affiliationMax-Planck-Institut für Molekulare Pflanzenphysiologie
cg.contributor.crpMaize
cg.contributor.donorCGIAR Trust Fund
cg.contributor.donorHarvestPlus
cg.contributor.initiativeAccelerated Breeding
cg.creator.identifierBabu Raman: 0000-0003-2610-6607
cg.creator.identifierDebra Skinner: 0000-0003-4715-670X
cg.creator.identifiernatalia palacios rojas: 0000-0002-3072-4611
cg.creator.identifierYunbi Xu: 0000-0003-3361-4650
cg.creator.identifierPrasanna Boddupalli: 0000-0002-5761-2273
cg.howPublishedFormally Published
cg.identifier.doihttps://doi.org/10.35196/rfm.2022.3.293
cg.isijournalISI Journal
cg.issn0187-7380
cg.issue3
cg.journalRevista Fitotecnia Mexicana
cg.number293
cg.placeMexico
cg.reviewStatusPeer Review
cg.subject.actionAreaGenetic Innovation
cg.subject.impactAreaNutrition, health and food security
cg.volume45
dc.contributor.authorHernández Rodríguez, Martha
dc.contributor.authorBabu, Raman
dc.contributor.authorSkinner, Debra J.
dc.contributor.authorPalacios Rojas, Natalia
dc.contributor.authorMartins, Marina C.M.
dc.contributor.authorGarcía Zavala, J. Jesús
dc.contributor.authorLobato Ortiz, Ricardo
dc.contributor.authorSantacruz Varela, Amalio
dc.contributor.authorCastillo-González, Fernando
dc.contributor.authorYunbi Xu
dc.contributor.authorBoddupalli, P.M.
dc.date.accessioned2023-01-03T14:32:35Zen
dc.date.available2023-01-03T14:32:35Zen
dc.identifier.urihttps://hdl.handle.net/10568/126507
dc.titleMarker development for quality protein maize breeding and an interaction study between Opaque-2 and Ask2 genesen
dcterms.abstractQuality Protein Maize (QPM) kernels contain twice the amounts of lysine and tryptophan compared to normal corn kernels. Although the opaque-2 (o2) mutation is the underlying cause of this beneficial change, other genes such as Aspartate kinase-2 (Ask2) affect the amino acid content in the endosperm to a lesser degree. To date, reports on the interaction between both loci are scarce and there are no high-throughput assays for the identification of the alleles of these genes. The objectives of this research were: 1) to study the interaction between the o2 and Ask2 genes with respect to the accumulation of amino acids in the endosperm in an F2 population, 2) to identify conserved SNPs into the o2 gene that can be used as markers, 3) to estimate the frequency of an SNP of Ask2 associated with the accumulation of lysine in the endosperm in CIMMYT germplasm, and 4) to develop high-throughput marker assays for these SNPs. The interaction study showed a preponderant effect of o2 on the accumulation of 11 amino acids (P ≤ 0.01). Ask2 appeared only to act with o2 to enhance marginally lysine, histidine and methionine levels in the double recessive homozygotes. Sequencing of amplicons at the o2 locus led to the identification of an SNP in exon 1 that discriminated all QPM (C) genotypes from non-QPM (T) genotypes. Validation of this SNP through KASP™ assays indicated that it was 92 % assertive in differentiating the o2 genotypes. In contrast, the frequency of the Ask2 SNP in CIMMYT QPM germplasm was low; however, an SSCP marker developed using this SNP detected five variants indicating that other unknown base changes may confer positive lysine-increasing responses. These markers could aid the marker-assisted selection of QPM cultivars.en
dcterms.accessRightsOpen Access
dcterms.audienceScientists
dcterms.available2022-09-15
dcterms.bibliographicCitationHernández-Rodríguez, M., Babu, R., Skinner, D. J., Palacios-Rojas, N., Martins, M. C. M., García-Zavala, J. J., Lobato-Ortiz, R., Santacruz-Varela, A., Castillo-González, F., Xu, Y., & Prasanna, B. M. (2022). DESARROLLO DE MARCADORES PARA MEJORAMIENTO DE MAÍZ CON PROTEÍNA DE CALIDAD Y ESTUDIO DE INTERACCIÓN ENTRE LOS GENES Opaco-2 Y Ask2. Revista Fitotecnia Mexicana, 45(3), 293. https://doi.org/10.35196/rfm.2022.3.293en
dcterms.issued2022-05-30
dcterms.languageen
dcterms.licenseCC-BY-NC-4.0
dcterms.publisherSociedad Mexicana de Fitogenetica A.C
dcterms.subjectzea maysen
dcterms.subjectmarker-assisted selectionen
dcterms.subjectsingle nucleotide polymorphismen
dcterms.subjectprotein qualityen
dcterms.typeJournal Article

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