A field survey of eight common potato pathogens in China based on integrated-loop-mediated isothermal amplification assays

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cg.authorship.typesCGIAR and developing country institute
cg.authorship.typesCGIAR and advanced research institute
cg.contributor.affiliationNanjing Agricultural University
cg.contributor.affiliationInternational Potato Center
cg.contributor.donorNational Key Research and Development Program
cg.coverage.countryChina
cg.coverage.iso3166-alpha2CN
cg.howPublishedFormally Published
cg.identifier.doihttps://doi.org/10.1186/s42483-024-00284-3
cg.isijournalISI Journal
cg.issn2524-4167
cg.issue1
cg.journalPhytopathology Research
cg.number62
cg.reviewStatusPeer Review
cg.subject.actionAreaGenetic Innovation
cg.subject.actionAreaResilient Agrifood Systems
cg.subject.cipPOTATOES
cg.subject.cipCROP PROTECTION
cg.subject.cipGENETICS, GENOMICS AND CROP IMPROVEMENT SCIENCES GGCI
cg.subject.cipFOOD SECURITY
cg.subject.impactAreaClimate adaptation and mitigation
cg.subject.impactAreaNutrition, health and food security
cg.subject.sdgSDG 2 - Zero hunger
cg.subject.sdgSDG 3 - Good health and well-being
cg.subject.sdgSDG 12 - Responsible consumption and production
cg.subject.sdgSDG 15 - Life on land
cg.volume6
dc.contributor.authorZhang, X.
dc.contributor.authorChen, H.
dc.contributor.authorWu, J.
dc.contributor.authorWang, X.
dc.contributor.authorWang, S.
dc.contributor.authorChen, J.
dc.contributor.authorYu, Q.
dc.contributor.authorZhang, Z.
dc.contributor.authorZheng, X.
dc.contributor.authorZeng, J.
dc.contributor.authorDong, S.
dc.date.accessioned2025-03-10T20:15:38Zen
dc.date.available2025-03-10T20:15:38Zen
dc.identifier.urihttps://hdl.handle.net/10568/173551
dc.titleA field survey of eight common potato pathogens in China based on integrated-loop-mediated isothermal amplification assaysen
dcterms.abstractVarious pathogens from oomycete, fungi, and bacteria kingdoms can infect potato and significantly reduce potato yield. The early diagnosis of potato pathogens is important for tracing disease epidemics and the subsequent disease management. Loop-mediated isothermal amplification (LAMP) is a critical technique for pathogen detection, but available LAMP assays do not effectively meet the requirement of field diagnoses due to complexities including co-infection of different pathogens. Hence, this study aims to develop integrated-LAMP assays (iLAMPs) for simultaneous detection of eight common potato pathogens and apply iLAMPs to pathogen detection in field samples from the four main potato-growing regions of China in 2023. Therefore, eight sets of primers showing gene- and genus-specificity were designed and used for iLAMPs to determine their specificity, sensitivity, and visualization. Subsequently, iLAMPs-mediated pathogen detection revealed that 72.82% of 206 diseased leaves and 84.94% of 239 diseased tubers carry more than one pathogen. The detection rate for each pathogen significantly varies from 1.94 to 65.53% in diseased leaves, and ranges from 26.78 to 52.72% in diseased tubers, respectively. In addition, the detection rate of Phytophthora infestans and Alternaria solani positively correlates in both leaves and tubers, especially for those samples from the southwestern and southern regions. Taken together, iLAMPs developed in this study enables simultaneous detection of eight common potato pathogens from field samples and may have broad applications in early management of potato diseases.en
dcterms.accessRightsOpen Access
dcterms.audienceAcademics
dcterms.audienceCGIAR
dcterms.audienceDevelopment Practitioners
dcterms.audienceDonors
dcterms.audienceExtension
dcterms.audienceFarmers
dcterms.audienceGeneral Public
dcterms.audienceNGOs
dcterms.audiencePolicy Makers
dcterms.audienceScientists
dcterms.available2024-12-10
dcterms.bibliographicCitationZhang, X.; Chen, H.; Wu, J.; Wang, X.; Wang, S.; Chen, J.; Yu, Q.; Zhang, Z.; Zheng, X.; Zeng, J.; Dong, S. 2024. A field survey of eight common potato pathogens in China based on integrated-loop-mediated isothermal amplification assays. Phytopathology Research. ISSN 2524-4167. 6(1). https://doi.org/10.1186/s42483-024-00284-3en
dcterms.extent14 p.
dcterms.issued2024-12-10
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherSpringer
dcterms.subjectsolanum tuberosumen
dcterms.subjectpotatoesen
dcterms.subjectplant protectionen
dcterms.typeJournal Article

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