A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen
| cg.identifier.doi | https://doi.org/10.1007/bf01092769 | |
| cg.isijournal | ISI Journal | |
| cg.issn | 0721-7714 | |
| cg.issn | 1432-203X | |
| cg.issue | 7 | |
| cg.journal | Plant Cell Reports | |
| cg.reviewStatus | Peer Review | |
| cg.subject.ciat | CASSAVA | |
| cg.volume | 16 | |
| dc.contributor.author | Escobar Pérez, Roosevelt H. | |
| dc.contributor.author | Mafla, G. | |
| dc.contributor.author | Roca, W. | |
| dc.date.accessioned | 2017-08-28T12:45:47Z | en |
| dc.date.available | 2017-08-28T12:45:47Z | en |
| dc.identifier.uri | https://hdl.handle.net/10568/83250 | |
| dc.title | A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen | en |
| dcterms.abstract | Shoot tips of in vitro-grown plantlets of cassava (Manihot esculenta Crantz), representing a wide range of germplasm, were cryopreserved as follows: precultured for 3 days, cryoprotected and dehydrated for 1 h, then frozen in liquid nitrogen using a six-step protocol. After 3 h in liquid nitrogen, the shoot tips were removed, rapidly warmed, and recultured sequentially in three recovery media. After 2 weeks, the regeneration of frozen shoot tips was completed. Genotypes with a low response were identified. Their response was attributed to the effects of pre- and post-freezing steps. Refining the methodology led to a consistent 50-70 percent plant recovery. (RA) | en |
| dcterms.accessRights | Limited Access | |
| dcterms.bibliographicCitation | Escobar Pérez, Roosevelt H.; Mafla B., Graciela; Roca, William M. 1997. A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen. Plant Cell Reports (Germany). 16(7):474-478. | en |
| dcterms.extent | p. 474-478 | |
| dcterms.issued | 1997 | |
| dcterms.language | en | |
| dcterms.license | Copyrighted; all rights reserved | |
| dcterms.publisher | Springer | |
| dcterms.subject | manihot esculenta | en |
| dcterms.subject | biological conservation | en |
| dcterms.subject | freezing | en |
| dcterms.subject | mandioca | en |
| dcterms.subject | conservación biológica | en |
| dcterms.subject | congelación | en |
| dcterms.type | Journal Article |