Reassociation kinetics-based approach for partial genome sequencing of the cattle tick, Rhipicephalus (Boophilus) microplus
Citation
Guerrero, F.D.; Moolhuijzen, P.; Peterson, D.G.; Bidwell, S.; Caler, E.; Bellgard, M.; Nene, V.M.; Djikeng, A. 2010. Reassociation kinetics-based approach for partial genome sequencing of the cattle tick, Rhipicephalus (Boophilus) microplus. BMC Genomics 11: 374.
Abstract/Description
The size and repetitive nature of the Rhipicephalus microplus genome makes obtaining a full genome sequence fiscally and technically problematic. To selectively obtain gene-enriched regions of this tick's genome, Cot filtration was performed, and Cot-filtered DNA was sequenced via 454 FLX pyrosequencing.
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Author ORCID identifiers
Vishvanath Nene https://orcid.org/0000-0001-7066-4169
Appolinaire Djikeng https://orcid.org/0000-0001-9271-3419
Appolinaire Djikeng https://orcid.org/0000-0001-9271-3419
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en
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Open Access