Investigating the diversity of plant-parasitic nematodes associated with coffee in Kenya, using morphological and molecular approaches

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cg.authorship.typesCGIAR and developing country institute
cg.contributor.affiliationGhent University
cg.contributor.affiliationUniversity of Florida
cg.contributor.affiliationVietnam Academy of Science and Technology
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.crpClimate Change, Agriculture and Food Security
cg.contributor.donorVlaamse Interuniversitaire Raad University Development Cooperation
cg.contributor.initiativePlant Health
cg.coverage.countryKenya
cg.coverage.iso3166-alpha2KE
cg.coverage.regionEastern Africa
cg.coverage.regionAfrica
cg.creator.identifierJoseph O. Maosa: 0000-0002-7833-3193
cg.creator.identifierZipporah Muthoni Githinji: 0009-0008-5714-9742
cg.creator.identifierDenis Gitonga: 0000-0001-8013-6482
cg.creator.identifierNGUYEN Huu Tien: 0000-0002-9476-935X
cg.creator.identifierMarjolein Couvreur: 0009-0007-6831-4400
cg.creator.identifierLAURA CORTADA: 0000-0002-5953-3798
cg.creator.identifierDaniel Coyne: 0000-0002-2030-6328
cg.creator.identifierWim Bert: 0000-0002-5864-412X
cg.howPublishedFormally Published
cg.identifier.doihttps://doi.org/10.1163/15685411-bja10438
cg.identifier.iitathemePLANT PRODUCTION & HEALTH
cg.identifier.projectIITA - Eastern Africa Hub
cg.isijournalISI Journal
cg.issn1388-5545
cg.issue9
cg.journalNematology
cg.reviewStatusPeer Review
cg.subject.actionAreaResilient Agrifood Systems
cg.subject.iitaCROP SYSTEMS
cg.subject.iitaBIOSCIENCE
cg.subject.iitaPLANT HEALTH
cg.subject.iitaPESTS OF PLANTS
cg.subject.iitaSOIL HEALTH
cg.subject.impactAreaNutrition, health and food security
cg.subject.impactPlatformNutrition, Health and Food Security
cg.subject.sdgSDG 2 - Zero hunger
cg.volume27
dc.contributor.authorMaosa, J.
dc.contributor.authorGithinji, Z.
dc.contributor.authorGitonga, D.
dc.contributor.authorNguyen, H.T.
dc.contributor.authorCouvreur, M.
dc.contributor.authorCortada, L.
dc.contributor.authorCoyne, D.L.
dc.contributor.authorBert, W.
dc.date.accessioned2025-11-12T21:57:59Z
dc.date.available2025-11-12T21:57:59Z
dc.identifier.urihttps://hdl.handle.net/10568/177855
dc.titleInvestigating the diversity of plant-parasitic nematodes associated with coffee in Kenya, using morphological and molecular approachesen
dcterms.abstractComprehensive surveys in nine coffee-growing counties in Kenya, involving 53 farms and comprising 116 soil and 63 root samples, revealed a total of 12 genera of plant-parasitic nematodes, including: Meloidogyne, Paratylenchus, Pratylenchus, Helicotylenchus, Rotylenchus, Rotylenchulus, Scutellonema, Trophotylenchulus, Xiphinema, Trichodorus, Aphelenchoides and Hemicycliophora. Meloidogyne was the most abundant genus, with densities of up to 210 juveniles (100 ml soil)− 1. Morphological and molecular characterisation enabled the identification of several nematode species including Meloidogyne javanica, M. africana, Rotylenchulus borealis and Trophotylenchulus obscurus. For T. obscurus, the 18S rDNA sequences obtained in this study are the first ever reported for the genus. Additionally, the 18S and D2-D3 of 28S, as well as ITS sequences, marked first for this species. In total, six partial 18S, seven D2-D3 of 28S, four ITS sequences of rDNA, ten COI, and two nad5 gene sequences were generated in this study. In conclusion, our study reveals that our knowledge of plant-parasitic nematode occurrence on important crops even, such as coffee, remains poorly documented and understood, highlighting the potential for new discoveries in such areas.en
dcterms.accessRightsLimited Access
dcterms.audienceScientists
dcterms.available2025-10-20
dcterms.bibliographicCitationMaosa, J., Githinji, Z., Gitonga, D., Nguyen, H.T., Couvreur, M., Cortada, L., ... & Bert, W. (2025). Investigating the diversity of plant-parasitic nematodes associated with coffee in Kenya, using morphological and molecular approaches. Nematology, 27(9), 1001-1018.
dcterms.extent1001-1018
dcterms.issued2025
dcterms.languageen
dcterms.licenseCopyrighted; all rights reserved
dcterms.publisherWalter de Gruyter GmbH
dcterms.subjecttaxonomy
dcterms.subjectphylogeny
dcterms.subjectcoffee
dcterms.subjectnematodes
dcterms.typeJournal Article

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