Genome-wide association for heat tolerance at seedling stage in historical spring wheat cultivars

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cg.authorship.typesCGIAR and developing country institute
cg.contributor.affiliationQuaid-i-Azam University
cg.contributor.affiliationBahauddin Zakariya University
cg.contributor.affiliationNational Agricultural Research Centre, Pakistan
cg.contributor.affiliationChinese Academy of Agricultural Sciences
cg.contributor.affiliationInternational Maize and Wheat Improvement Center
cg.contributor.donorNational Science Foundation of China
cg.contributor.donorCGIAR Trust Fund
cg.contributor.donorHainan Yazhou Bay Seed Lab, China
cg.contributor.initiativeAccelerated Breeding
cg.creator.identifierawais rasheed: 0000-0003-2528-708X
cg.creator.identifierHuihui Li: 0000-0002-9117-5011
cg.howPublishedFormally Published
cg.identifier.doihttps://doi.org/10.3389/fpls.2022.972481
cg.isijournalISI Journal
cg.issn1664-462X
cg.journalFrontiers in Plant Science
cg.number972481
cg.reviewStatusPeer Review
cg.subject.actionAreaGenetic Innovation
cg.subject.impactAreaNutrition, health and food security
cg.subject.impactAreaClimate adaptation and mitigation
cg.volume13
dc.contributor.authorKhan, Muhammad Ibrar
dc.contributor.authorKainat, Zarnishal
dc.contributor.authorMaqbool, Saman
dc.contributor.authorMehwish, Ambreen
dc.contributor.authorAhmad, Suhaib
dc.contributor.authorHafiz Muhammad Suleman
dc.contributor.authorMahmood, Zahid
dc.contributor.authorAli, Mohsin
dc.contributor.authorAziz, Abdul
dc.contributor.authorRasheed, Awais
dc.contributor.authorHuihui Li
dc.date.accessioned2023-01-25T20:02:44Zen
dc.date.available2023-01-25T20:02:44Zen
dc.identifier.urihttps://hdl.handle.net/10568/128239
dc.titleGenome-wide association for heat tolerance at seedling stage in historical spring wheat cultivarsen
dcterms.abstractIncreasing global temperature has adverse effects on crop health and productivity at both seedling and reproductivity stages. It is paramount to develop heat tolerant wheat cultivars able to sustain under high and fluctuating temperature conditions. An experiment was conducted to characterize 194 historical wheat cultivars of Pakistan under high temperature at seedling stage to identify loci associated with heat tolerance using genome-wide association studies (GWAS). A quantitative trait locus, TaHST1, on chr4A was also characterized to identify the haplotypes at this locus associated with heat tolerance in wheat from Pakistan. Initially, the diversity panel was planted under control conditions (25°C/20°C day and night temperature) in a glass house. At three leaf stage, plants were subjected to heat stress (HS) by increasing temperature (40°C/35°C day and night), while one treatment was kept at control condition. After 7 days of HS, data were collected for seedling morphology. Heat stress reduced these traits by 25% (root weight) to 40% (shoot weight), and shoot biomass was largely affected by heat stress. A GWAS model, fixed and random model circulating probability unification (FarmCPU), identified 43 quantitative trait nucleotides (QTNs) on all chromosomes, except chr7B, were associated under both HS and control conditions. Thirteen QTNs were identified in control, while 30 QTNs were identified in HS condition. In total, 24 haplotypes were identified at TaHST1 locus, and most of the heat tolerant genotypes were assigned to Hap-20 and Hap-21. Eleven QTNs were identified within 0.3–3.1 Mb proximity of heat shock protein (HSP). Conclusively, this study provided a detailed genetic framework of heat tolerance in wheat at the seedling stage and identify potential genetic regions associated with heat tolerance which can be used for marker assisted selection (MAS) in breeding for heat stress tolerance.en
dcterms.accessRightsOpen Access
dcterms.audienceScientists
dcterms.available2022-08-25
dcterms.bibliographicCitationKhan, M. I., Kainat, Z., Maqbool, S., Mehwish, A., Ahmad, S., Suleman, H. M., Mahmood, Z., Ali, M., Aziz, A., Rasheed, A., & Li, H. (2022). Genome-wide association for heat tolerance at seedling stage in historical spring wheat cultivars. Frontiers in Plant Science, 13. https://doi.org/10.3389/fpls.2022.972481en
dcterms.issued2022-08-25
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherFrontiers Media
dcterms.subjectheat stressen
dcterms.subjectquantitative trait locien
dcterms.subjectnucleotidesen
dcterms.subjectsingle nucleotide polymorphismen
dcterms.subjectspring wheaten
dcterms.typeJournal Article

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