Genome and systems biology of Melilotus albus provides insights into coumarins biosynthesis

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cg.authorship.typesCGIAR and developing country institute
cg.authorship.typesCGIAR and advanced research institute
cg.contributor.affiliationLanzhou University
cg.contributor.affiliationAgResearch, New Zealand
cg.contributor.affiliationInternational Livestock Research Institute
cg.contributor.donorNational Natural Science Foundation of China
cg.creator.identifierChris Jones: 0000-0001-9096-9728
cg.howPublishedFormally Published
cg.identifier.doihttps://doi.org/10.1111/pbi.13742
cg.isijournalISI Journal
cg.issn1467-7652
cg.issue3
cg.journalPlant Biotechnology Journal
cg.reviewStatusPeer Review
cg.subject.ilriBIOTECHNOLOGY
cg.subject.ilriCROPS
cg.subject.ilriFARMING SYSTEMS
cg.volume20
dc.contributor.authorFan Wu
dc.contributor.authorZhen Duan
dc.contributor.authorPan Xu
dc.contributor.authorQi Yan
dc.contributor.authorMinghui Meng
dc.contributor.authorCao, M.
dc.contributor.authorJones, Christopher S.
dc.contributor.authorXifang Zong
dc.contributor.authorPei Zhou
dc.contributor.authorYimeng Wang
dc.contributor.authorKai Luo
dc.contributor.authorShengsheng Wang
dc.contributor.authorZhuanzhuan Yan
dc.contributor.authorPenglei Wang
dc.contributor.authorHongyan Di
dc.contributor.authorZifeng Ouyang
dc.contributor.authorYanrong Wang
dc.contributor.authorJiyu Zhang
dc.date.accessioned2021-11-08T10:43:12Zen
dc.date.available2021-11-08T10:43:12Zen
dc.identifier.urihttps://hdl.handle.net/10568/115873
dc.titleGenome and systems biology of Melilotus albus provides insights into coumarins biosynthesisen
dcterms.abstractMelilotus species are used as green manure and rotation crops worldwide and contain abundant pharmacologically active coumarins. However, there is a paucity of information on its genome and coumarin production and function. Here, we reported a chromosome‐scale assembly of Melilotus albus genome with 1.04 Gb in eight chromosomes, containing 71.42% repetitive elements. Long terminal repeat retrotransposon bursts coincided with declining of population sizes during the Quaternary glaciation. Resequencing of 94 accessions enabled insights into genetic diversity, population structure, and introgression. Melilotus officinalis had relatively larger genetic diversity than that of M. albus. The introgression existed between M. officinalis group and M. albus group, and gene flows was from M. albus to M. officinalis. Selection sweep analysis identified candidate genes associated with flower colour and coumarin biosynthesis. Combining genomics, BSA, transcriptomics, metabolomics, and biochemistry, we identified a β‐glucosidase (BGLU) gene cluster contributing to coumarin biosynthesis. MaBGLU1 function was verified by overexpression in M. albus, heterologous expression in Escherichia coli, and substrate feeding, revealing its role in scopoletin (coumarin derivative) production and showing that nonsynonymous variation drives BGLU enzyme activity divergence in Melilotus. Our work will accelerate the understanding of biologically active coumarins and their biosynthetic pathways, and contribute to genomics‐enabled Melilotus breeding.en
dcterms.accessRightsOpen Access
dcterms.audienceScientists
dcterms.available2021-11-16
dcterms.bibliographicCitationFan Wu, Zhen Duan, Pan Xu, Qi Yan, Minghui Meng, Cao, M., Jones, C.S., Xifang Zong, Pei Zhou, Yimeng Wang, Kai Luo, Shengsheng Wang, Zhuanzhuan Yan, Penglei Wang, Hongyan Di, Zifeng Ouyang, Yanrong Wang and Jiyu Zhang. 2021. Genome and systems biology of Melilotus albus provides insights into coumarins biosynthesis. Plant Biotechnology Journalen
dcterms.extentpp. 592-609
dcterms.issued2022-03
dcterms.languageen
dcterms.licenseCC-BY-NC-ND-4.0
dcterms.publisherWiley
dcterms.subjectbiotechnologyen
dcterms.subjectmelilotus albaen
dcterms.subjectplant biotechnologyen
dcterms.subjectcropsen
dcterms.typeJournal Article

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