Post transcriptional silencing of reporter transgenes in tobacco correlates whit DNA methylation

Ingelbrecht, I., Van Houdt, H., Van Montagu, M. & Depicker, A. (1994). Post-transcriptional silencing of reporter transgenes in tobacco correlates whit DNA methylation. In Plant Biology: Proceedings of the National Academy of Sciences of the United States of America, 91(22), (p. 10502-10506).

Endogenous plant genes or transgenes can be silenced on introduction of homologous gene sequences. Here we document a reporter gene-silencing event in Nicotiana tabacum that has a distinctive combination of features--i.e., (i) silencing occurs by a posttranscriptional process, (ii) silencing correlates with DNA methylation, and (iii) this de novo methylation is not restricted to cytosines located in the symmetrical motifs CG and CXG.