A case study from the overexpression of OsTZF5, encoding a CCCH tandem zinc finger protein, in rice plants across nineteen yield trials

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cg.authorship.typesCGIAR and developing country institute
cg.authorship.typesCGIAR and advanced research institute
cg.authorship.typesCGIAR single centre
cg.contributor.affiliationJapan International Research Center for Agricultural Sciences
cg.contributor.affiliationUniversité de Montpellier
cg.contributor.affiliationInternational Rice Research Institute
cg.contributor.affiliationThe University of Agriculture, Peshawar
cg.contributor.affiliationUniversity of California
cg.contributor.affiliationUniversity of Tokyo
cg.contributor.affiliationResearch Institute for Agricultural and Life Sciences
cg.contributor.donorMinistry of Agriculture, Forestry and Fisheries, Japan
cg.contributor.donorCGIAR Trust Fund
cg.contributor.initiativeAccelerated Breeding
cg.coverage.regionAsia
cg.coverage.regionAmericas
cg.creator.identifierAlexandre Grondin: 0000-0001-6726-6274
cg.creator.identifierMignon Natividad: 0000-0002-4007-8035
cg.creator.identifierTakuya Ogata: 0000-0003-3361-6234
cg.creator.identifierAmelie Gaudin: 0000-0003-4007-9991
cg.creator.identifierKurniawan Trijatmiko: 0000-0002-6440-9150
cg.creator.identifierKyonoshin Maruyama: 0000-0001-7725-0380
cg.creator.identifierYasunari Fujita: 0000-0002-5036-8319
cg.creator.identifierKazuko Yamaguchi-Shinozaki: 0000-0002-0249-8258
cg.creator.identifierInez Slamet-Loedin: 0000-0001-9145-3571
cg.creator.identifierAmelia Henry: 0000-0001-6255-5480
cg.edition2024
cg.howPublishedFormally Published
cg.identifier.doihttps://doi.org/10.1186/s12284-024-00705-z
cg.isijournalISI Journal
cg.issn1939-8425
cg.issue1
cg.journalRice
cg.number25
cg.reviewStatusPeer Review
cg.subject.actionAreaGenetic Innovation
cg.subject.impactAreaNutrition, health and food security
cg.volume17
dc.contributor.authorGrondin, Alexandre
dc.contributor.authorNatividad, Mignon A.
dc.contributor.authorOgata, Takuya
dc.contributor.authorJan, Asad
dc.contributor.authorGaudin, Amélie C. M.
dc.contributor.authorTrijatmiko, Kurniawan R.
dc.contributor.authorLiwanag, Evelyn
dc.contributor.authorMaruyama, Kyonoshin
dc.contributor.authorFujita, Yasunari
dc.contributor.authorYamaguchi-Shinozaki, Kazuko
dc.contributor.authorNakashima, Kazuo
dc.contributor.authorSlamet-Loedin, Inez H.
dc.contributor.authorHenry, Amelia
dc.date.accessioned2024-12-05T20:44:25Zen
dc.date.available2024-12-05T20:44:25Zen
dc.identifier.urihttps://hdl.handle.net/10568/163109
dc.titleA case study from the overexpression of OsTZF5, encoding a CCCH tandem zinc finger protein, in rice plants across nineteen yield trialsen
dcterms.abstractBackground Development of transgenic rice overexpressing transcription factors involved in drought response has been previously reported to confer drought tolerance and therefore represents a means of crop improvement. We transformed lowland rice IR64 with OsTZF5, encoding a CCCH-tandem zinc finger protein, under the control of the rice LIP9 stress-inducible promoter and compared the drought response of transgenic lines and nulls to IR64 in successive screenhouse paddy and field trials up to the T6 generation. Results Compared to the well-watered conditions, the level of drought stress across experiments varied from a minimum of − 25 to − 75 kPa at a soil depth of 30 cm which reduced biomass by 30–55% and grain yield by 1–92%, presenting a range of drought severities. OsTZF5 transgenic lines showed high yield advantage under drought over IR64 in early generations, which was related to shorter time to flowering, lower shoot biomass and higher harvest index. However, the increases in values for yield and related traits in the transgenics became smaller over successive generations despite continued detection of drought-induced transgene expression as conferred by the LIP9 promoter. The decreased advantage of the transgenics over generations tended to coincide with increased levels of homozygosity. Background cleaning of the transgenic lines as well as introgression of the transgene into an IR64 line containing major-effect drought yield QTLs, which were evaluated starting at the BC3F1 and BC2F3 generation, respectively, did not result in consistently increased yield under drought as compared to the respective checks. Conclusions Although we cannot conclusively explain the genetic factors behind the loss of yield advantage of the transgenics under drought across generations, our results help in distinguishing among potential drought tolerance mechanisms related to effectiveness of the transgenics, since early flowering and harvest index most closely reflected the levels of yield advantage in the transgenics across generations while reduced biomass did not.en
dcterms.accessRightsOpen Access
dcterms.audienceCGIAR
dcterms.audienceDonors
dcterms.audienceScientists
dcterms.available2024-04-09
dcterms.bibliographicCitationGrondin, A., Natividad, M.A., Ogata, T. et al. A Case Study from the Overexpression of OsTZF5, Encoding a CCCH Tandem Zinc Finger Protein, in Rice Plants Across Nineteen Yield Trials. Rice 17, 25 (2024). https://doi.org/10.1186/s12284-024-00705-zen
dcterms.issued2024-12
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherSpringer
dcterms.subjecttransgenicsen
dcterms.subjecttransgenesen
dcterms.subjectriceen
dcterms.subjectdroughten
dcterms.subjectyieldsen
dcterms.typeJournal Article

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