PCR markerbased analysis of wild and cultivated yams (Dioscorea spp.)in Nigeria: genetic relationships and implication for ex situ conservation

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cg.authorship.typesCGIAR and developing country institute
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationVirginia Polytechnic Institute and State University
cg.contributor.affiliationInstitut de Recherche Agricole pour le Développement, Cameroon
cg.contributor.affiliationMahyco Research Foundation, India
cg.coverage.countryNigeria
cg.coverage.iso3166-alpha2NG
cg.coverage.regionAfrica
cg.coverage.regionWestern Africa
cg.identifier.doihttps://doi.org/10.1007/s10722-004-6128-7
cg.isijournalISI Journal
cg.issn0925-9864
cg.issue6
cg.journalGenetic Resources and Crop Evolution
cg.reviewStatusPeer Review
cg.subject.iitaYAM
cg.subject.iitaGENETIC IMPROVEMENT
cg.subject.iitaPLANT BREEDING
cg.subject.iitaPLANT PRODUCTION
cg.volume52
dc.contributor.authorMignouna, H.
dc.contributor.authorAbang, Mathew M.
dc.contributor.authorWanyera, N.
dc.contributor.authorChikaleke, V.
dc.contributor.authorAsiedu, Robert
dc.contributor.authorThottappilly, G.
dc.date.accessioned2018-03-23T06:48:46Zen
dc.date.available2018-03-23T06:48:46Zen
dc.identifier.urihttps://hdl.handle.net/10568/91792
dc.titlePCR markerbased analysis of wild and cultivated yams (Dioscorea spp.)in Nigeria: genetic relationships and implication for ex situ conservationen
dcterms.abstractReliable characterization of the variation among wild and cultivated yams in Nigeria is essential for improved management and efficient utilization of yam genetic resources. RAPD and double stringency PCR (DS-PCR) analyses were used to investigate genetic relationships and the extent of redundancy among 30 accessions of two cultivated, and 35 accessions of four wild yam species collected from Nigeria. Twenty-five selected random decamer and two microsatellite primers were used individually and in combination to generate DNA profiles for each accession of the six Dioscorea species. The number of amplified fragments varied from 7 to 18 fragments per primer/primer combination. Different levels of intraspecific genetic diversity were found, with Dioscorea rotundata Poir. being the most variable. Based on identical profiles for the RAPD and DS-PCR primers, 12 duplication groups consisting of a total number of 37 accessions were observed in the present study. An UPGMA analysis grouped the majority of plants according to the species. Cultivated yams belonging to the D. cayenensis–rotundata species complex, which were classified into seven morphotypes/varietal groups, could be clearly separated into two major groups corresponding to D. rotundata Poir. and D. cayenensis Lam. D. cayenensis cultivars exhibited a low level of intraspecific variation and were genetically close to the wild species Dioscorea burkilliana J. Miège. D. rotundata cultivars classified into six varietal groups showed a high degree of DNA polymorphism and were separated into two major groups that appeared most closely related to Dioscorea praehensilis Benth. and Dioscorea liebrechtsiana de Wild. We propose, based on these results, that cultivars classified into D. cayenensis should be considered as a taxon separate from D. rotundata. The implications of intraspecific variability for the ex situ conservation of wild and cultivated yam germplasm in Nigeria are discussed.en
dcterms.accessRightsLimited Access
dcterms.bibliographicCitationMignouna, H., Abang, M., Wanyera, N., Chikaleke, V., Asiedu, R. & Thottappilly, G. (2005). PCR marker-based analysis of wild and cultivated yams (Dioscorea spp.) in Nigeria: genetic relationships and implications for ex situ conservation. Genetic Resources and Crop Evolution, 52(6), 755-763.en
dcterms.extentp. 755-763
dcterms.issued2005-09
dcterms.languageen
dcterms.licenseCopyrighted; all rights reserved
dcterms.publisherSpringer
dcterms.subjectdioscoreaen
dcterms.subjectgenetic resourcesen
dcterms.subjectguinea yamsen
dcterms.subjectrapden
dcterms.subjectvariabilityen
dcterms.subjectwild yamen
dcterms.typeJournal Article

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