Diversity of Meloidogyne spp. from peri-urban areas of sub-Saharan Africa and their genetic similarity with populations from the Latin America

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cg.authorship.typesCGIAR and developing country instituteen
cg.contributor.affiliationEmpresa Brasileira de Pesquisa Agropecuáriaen
cg.contributor.affiliationUniversity of Brasíliaen
cg.contributor.affiliationUniversity of Côte d'Azuren
cg.contributor.affiliationInternational Institute of Tropical Agricultureen
cg.contributor.crpRoots, Tubers and Bananas
cg.coverage.countryBenin
cg.coverage.countryKenya
cg.coverage.countryNigeria
cg.coverage.countryTanzania
cg.coverage.countryUganda
cg.coverage.iso3166-alpha2BJ
cg.coverage.iso3166-alpha2KE
cg.coverage.iso3166-alpha2NG
cg.coverage.iso3166-alpha2TZ
cg.coverage.iso3166-alpha2UG
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.coverage.regionWestern Africa
cg.creator.identifierDaniel Coyne: 0000-0002-2030-6328
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1016/j.pmpp.2018.08.004en
cg.identifier.iitathemeNATURAL RESOURCE MANAGEMENT
cg.isijournalISI Journalen
cg.issn0885-5765en
cg.journalPhysiological and Molecular Plant Pathologyen
cg.reviewStatusPeer Reviewen
cg.subject.iitaNATURAL RESOURCE MANAGEMENTen
cg.volume105en
dc.contributor.authorSantos, M.F. dosen
dc.contributor.authorSilva Mattos, V. daen
dc.contributor.authorMonteiro, J.M.en
dc.contributor.authorAlmeida, M.R.en
dc.contributor.authorJorge, A.S.en
dc.contributor.authorCares, J.E.en
dc.contributor.authorCastagnone-Sereno, P.en
dc.contributor.authorCoyne, D.en
dc.contributor.authorCarneiro, R.M.en
dc.date.accessioned2019-02-08T10:07:17Zen
dc.date.available2019-02-08T10:07:17Zen
dc.identifier.urihttps://hdl.handle.net/10568/99340
dc.titleDiversity of Meloidogyne spp. from peri-urban areas of sub-Saharan Africa and their genetic similarity with populations from the Latin Americaen
dcterms.abstractIn Africa, peri-urban vegetable production systems supply perishable vegetables to the rapidly expanding urban centers. These highly intensive systems are characterized by high levels of pests and diseases and an excessive use of synthetic pesticides to reduce their population densities. Root-knot nematodes (RKN) are especially prevalent in these systems but are often not recognized, or diagnosed correctly. The limited ability to accurately identify these pathogens likely results in the inappropriate use and misuse of control measures, such as genetic resistance, crop rotation, or synthetic chemicals. Given the perceived importance of RKN, a species characterization study was conducted in peri-urban vegetable (amaranthus, cabbage, pepper, carrot, cassava, eggplant, okra, tomato) fields and some coffee plantations, in Benin, Kenya, Nigeria, Tanzania and Uganda. Meloidogyne spp. were characterized from 143 field samples using esterase phenotypes (EST) and SCAR markers. Five known species were identified: three phenotypes for M. javanica populations (EST J3, Rm: 1.0, 1.25, 1.4; EST J2a, Rm: 1.0, 1.4; EST J2b, Rm: 1.0, 1.25), two for M. incognita (EST I1, Rm: 1.0; EST I2, Rm: 1.05, 1.0), one for M. arenaria (EST A2, Rm: 1.2, 1.3), one for M. enterolobii (EST E4, Rm: 0.70, 0.75, 0.90, 0.95), one for M. izalcoensis (EST I4 Rm: 0.86, 0.96, 1.24, 1.30) and two unusual esterase phenotypes for two unknown species, named Meloidogyne sp.1 and sp.2. Combinations of species were detected from numerous locations. Genetic diversity was further studied using RAPD primers, by comparing a subset of the sampled populations from Africa and some populations from Brazil and El Salvador. The analysis identified separate clusters of the more common and minor species, with low variability observed for African and American populations. The SCAR markers correctly identified all Meloidogyne species with the exception of M. ethiopica, Meloidogyne sp.1 and Meloidogyne sp.2. For Meloidogyne sp.1, the SCAR markers corresponded wrongly to M. javanica and M. arenaria, and for Meloidogyne sp.2 to M. incognita. This demonstrates the shortcomings of using SCAR markers alone, which can generate erroneous results for RKN species. Further morphological and molecular studies are required to clarify the identity of these two atypical species.en
dcterms.accessRightsLimited Access
dcterms.audienceScientistsen
dcterms.bibliographicCitationdos Santos, M.F., da Silva Mattos, V., Monteiro, J.M., Almeida, M.R., Jorge Jr, A.S., Cares, J.E., ... & Carneiro, R.M. (2018). Diversity of Meloidogyne spp. from peri-urban areas of sub-Saharan Africa and their genetic similarity with populations from the Latin America. Physiological and Molecular Plant Pathology, 105, 110-118.en
dcterms.extent110-118en
dcterms.issued2019-01
dcterms.languageen
dcterms.licenseCopyrighted; all rights reserved
dcterms.publisherElsevieren
dcterms.subjectesteraseen
dcterms.subjectphenotypesen
dcterms.subjectspeciesen
dcterms.subjectidentificationen
dcterms.subjectrapden
dcterms.subjectcrypticen
dcterms.typeJournal Article

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