Proof of concept and early development stage of market-oriented high iron and zinc rice expressing dicot ferritin and rice nicotianamine synthase genes

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cg.authorship.typesCGIAR single centre
cg.contributor.crpAgriculture for Nutrition and Health
cg.contributor.donorBill & Melinda Gates Foundation
cg.coverage.countryBangladesh
cg.coverage.countryPhilippines
cg.coverage.iso3166-alpha2BD
cg.coverage.iso3166-alpha2PH
cg.coverage.regionSouthern Asia
cg.coverage.regionAsia
cg.coverage.regionSouth-eastern Asia
cg.creator.identifierHowarth Bouis: 0000-0001-5432-9192
cg.identifier.doihttps://doi.org/10.1038/s41598-022-26854-z
cg.identifier.projectIFPRI - Director General's Office
cg.identifier.projectIFPRI - HarvestPlus
cg.identifier.publicationRankA
cg.isijournalISI Journal
cg.issn2045-2322
cg.issue1
cg.journalScientific Reports
cg.number676
cg.reviewStatusPeer Review
cg.volume13
dc.contributor.authorTsakirpaloglou, Nikolaos
dc.contributor.authorBueno-Mota, Gela
dc.contributor.authorSoriano, Jessica Candace
dc.contributor.authorArcillas, Erwin
dc.contributor.authorYu, Su-May
dc.contributor.authorBouis, Howarth E.
dc.contributor.authorArines, Felichi Mae
dc.contributor.authorStangoulis, James
dc.contributor.authorTrijatmiko, Kurniawan Rudi
dc.contributor.authorReinke, Russell
dc.contributor.authorTohme, Joseph
dc.contributor.authorSlamet-Loedin, Inez H.
dc.date.accessioned2024-03-14T12:08:49Zen
dc.date.available2024-03-14T12:08:49Zen
dc.identifier.urihttps://hdl.handle.net/10568/140019
dc.titleProof of concept and early development stage of market-oriented high iron and zinc rice expressing dicot ferritin and rice nicotianamine synthase genesen
dcterms.abstractMicronutrient deficiencies such as iron (Fe), zinc (Zn), and vitamin A, constitute a severe global public health phenomenon. Over half of preschool children and two-thirds of nonpregnant women of reproductive age worldwide have micronutrient deficiencies. Biofortification is a cost-effective strategy that comprises a meaningful and sustainable means of addressing this issue by delivering micronutrients through staple foods to populations with limited access to diverse diets and other nutritional interventions. Here, we report on the proof-of-concept and early development stage of a collection of biofortified rice events with a high density of Fe and Zn in polished grains that have been pursued further to advance development for product release. In total, eight constructs were developed specifically expressing dicot ferritins and the rice nicotianamine synthase 2 (OsNAS2) gene under different combinations of promoters. A large-scale transformation of these constructs to Bangladesh and Philippines commercial indica cultivars and subsequent molecular screening and confined field evaluations resulted in the identification of a pool of ten events with Fe and Zn concentrations in polished grains of up to 11 μg g−1 and up to 37 μg g−1, respectively. The latter has the potential to reduce the prevalence of inadequate Zn intake for women of childbearing age in Bangladesh and in the Philippines by 30% and 50%, respectively, compared to the current prevalence. To our knowledge, this is the first potential biotechnology public-sector product that adopts the product cycle phase-gated approach, routinely applied in the private sector.en
dcterms.accessRightsOpen Access
dcterms.available2023-01-12
dcterms.bibliographicCitationTsakirpaloglou, Nikolaos; Bueno-Mota, Gela; Soriano, Jessica Candace; Arcillas, Erwin; Yu, Su-May, Bouis, Howarth; et al. 2023. Proof of concept and early development stage of market-oriented high iron and zinc rice expressing dicot ferritin and rice nicotianamine synthase genes Scientific Reports 13: 676. https://doi.org/10.1038/s41598-022-26854-zen
dcterms.issued2023-01-12
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherNature Publishing Group
dcterms.replaceshttps://ebrary.ifpri.org/digital/collection/p15738coll5/id/8558
dcterms.subjectrice polishingsen
dcterms.subjectbiofortificationen
dcterms.subjectriceen
dcterms.subjectretinolen
dcterms.subjecttrace elementsen
dcterms.subjectchildrenen
dcterms.subjectironen
dcterms.subjectzincen
dcterms.subjectpublic healthen
dcterms.subjectwomenen
dcterms.typeJournal Article

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