Multi-omics analysis reveals the roles of purple acid phosphatases in organic phosphorus utilization by the tropical legume Stylosanthes guianensis

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cg.authorship.typesCGIAR and advanced research institute
cg.contributor.affiliationChinese Academy of Tropical Agricultural Sciences
cg.contributor.affiliationHainan University
cg.contributor.affiliationInternational Center for Tropical Agriculture
cg.contributor.donorCGIAR Trust Fund
cg.contributor.initiativeLivestock and Climate
cg.creator.identifierJacobo Arango: 0000-0002-4828-9398
cg.creator.identifierIdupulapati M. Rao: 0000-0002-8381-9358
cg.creator.identifierRainer Schultze-Kraft: 0000-0002-4563-0044
cg.creator.identifierGuodao Liu: 0000-0003-4189-9959
cg.creator.identifierPandao Liu: 0000-0003-2296-9130
cg.identifier.doihttps://doi.org/10.1111/tpj.16526
cg.isijournalISI Journal
cg.issn0960-7412
cg.issn1365-313X
cg.issue3
cg.journalThe Plant Journal
cg.reviewStatusPeer Review
cg.subject.alliancebiovciatTROPICAL FORAGES
cg.volume117
dc.contributor.authorLuo, Jiajia
dc.contributor.authorChen, Zhijian
dc.contributor.authorHuang, Rui
dc.contributor.authorWu, Yuanhang
dc.contributor.authorLiu, Chun
dc.contributor.authorCai, Zeping
dc.contributor.authorDong, Rongshu
dc.contributor.authorArango, Jacobo
dc.contributor.authorRao, Idupulapati M.
dc.contributor.authorSchultze-Kraft, Rainer
dc.contributor.authorLiu, Guodao
dc.contributor.authorLiu, Pandao
dc.date.accessioned2023-12-04T10:18:29Zen
dc.date.available2023-12-04T10:18:29Zen
dc.identifier.urihttps://hdl.handle.net/10568/134939
dc.titleMulti-omics analysis reveals the roles of purple acid phosphatases in organic phosphorus utilization by the tropical legume Stylosanthes guianensisen
dcterms.abstractStylo (Stylosanthes guianensis) is a tropical legume known for its exceptional tolerance to low phosphate (Pi), a trait believed to be linked to its high acid phosphatase (APase) activity. Previous studies have observed genotypic variations in APase activity in stylo; however, the gene encoding the crucial APase responsible for this variation remains unidentified. In this study, transcriptomic and proteomic analyses were employed to identify eight Pi starvation‐inducible (PSI) APases belonging to the purple APase (PAP) family in the roots of stylo and seven in the leaves. Among these PSI‐PAPs, SgPAP7 exhibited a significantly positive correlation in its expression levels with the activities of both internal APase and root‐associated APase across 20 stylo genotypes under low‐Pi conditions. Furthermore, the recombinant SgPAP7 displayed high catalytic activity toward adenosine 5′‐diphosphate (ADP) and phosphoenolpyruvate (PEP) in vitro. Overexpression (OE) of SgPAP7 in Arabidopsis facilitated exogenous organic phosphorus utilization. Moreover, SgPAP7 OE lines showed lower shoot ADP and PEP levels than the wild type, implying that SgPAP7 is involved in the catabolism and recycling of endogenous ADP and PEP, which could be beneficial for plant growth in low‐Pi soils. In conclusion, SgPAP7 is a key gene with a major role in stylo adaptation to low‐Pi conditions by facilitating the utilization of both exogenous and endogenous organic phosphorus sources. It may also function as a PEP phosphatase involved in a glycolytic bypass pathway that minimizes the need for adenylates and Pi. Thus, SgPAP7 could be a promising target for improving tolerance of crops to low‐Pi availability.en
dcterms.accessRightsOpen Access
dcterms.available2023-11-06
dcterms.bibliographicCitationLuo, J.; Chen, Z.; Huang, R.; Wu, Y.; Liu, C.; Cai, Z.; Dong, R.; Arango, J.; Rao, I.M.; Schultze-Kraft, R.; Liu, G. and Liu, P. 2024. Multi-omics analysis reveals the roles of purple acid phosphatases in or-ganic phosphorus utilization by the tropical legume Stylosanthes guianensis. The Plant Journal 117(3):729-746. ISSN: 1365-313Xen
dcterms.extentpp. 729-746
dcterms.issued2024-02
dcterms.languageen
dcterms.licenseCopyrighted; all rights reserved
dcterms.publisherWiley
dcterms.subjectphosphorusen
dcterms.subjectusesen
dcterms.subjectproteomicsen
dcterms.subjecttranscriptomicsen
dcterms.subjectanalysisen
dcterms.subjecttropical legumesen
dcterms.subjectstylosanthes guianensisen
dcterms.typeJournal Article

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